Table 1.
Biological activities of the peptide fractions separated by precipitation, ion-exchange and reverse-phase chromatography of 2-h egg yolk protein by-product (YP) hydrolysate treated by pepsin
| Isolation step | Sample | ACE IC50 (µg/mL) | DPPH (µM Troloxeq/mg) | FRAP (µg Fe2+/m) | CHEL (µg Fe2+/mg) |
|---|---|---|---|---|---|
| Hydrolysis | Peptic hydrolysate (2 h) (DH 45.3 %) | 623.0a | 0.5h,i | 56.4f | 405.0b |
| Precipitation (pH 4.0; centrifugation) | Supernatant | 361.0d | 1.6d,e | 78.1d | 458.6a |
| Ion-exchange chromatography | 1 | 249.1e | 0.54hi | 70.5e | 13.2h |
| 2 | 291.5d | 0.55hi | 23.3i | 94.6f | |
| 3 | NA | 1.5d | 15.1j | 109.3f | |
| 4 | 283.0d | 2.9b | 110.6c | 250.1c | |
| 5 | 475.8b | 1.4e | NA | 128.2e | |
| 6 | NA | 0.67gh | NA | 38.3g | |
| RP-HPLC | A | NA | NA | NA | NA |
| B | 152.3f | 1.9c | 112.1c | NA | |
| C (4.C) | 35.0h | 3.0ab | 184.2b | 406.5b | |
| D | 72.7g | 0.8g | 76.3de | 175.6d | |
| E | 245.9e | 0.8g | 54.7f | NA | |
| F | 423.0c | 1.0f | 40.0g | NA | |
| G | NA | NA | 31.2h | NA | |
| H | NA | 0.4i | 25.6hi | 27.3gh | |
| 4.C.1 | 29.0h | 3.1a | 190.8a | 402.5b |
All data were expressed as mean values (n = 3)
Values sharing the same letter are not significantly different at p < 0.05
Mean values with different letters in the same column are significantly different at p ≤ 0.05
NA no activity