Fig. 4.

Comparison of the effect of antipsychotic medications with ITI-007 on the phosphorylation state of striatal TH in vivo. Mice (N = 6/treatment group) were treated acutely with behaviorally efficacious doses of ITI-007 (3 mg/kg, p.o.), clozapine (5 mg/kg, i.p.), aripiprazole (10 mg/kg, p.o.), quetiapine (10 mg/kg, i.p.), olanzapine (1 mg/kg, i.p.), risperidone (3 mg/kg, p.o.), or haloperidol (1 mg/kg, i.p.) then killed 15, 30, or 60 min later. The change in phosphorylation state at serine (S) 40 of tyrosine hydroxylase (TH) was determined in striatal samples by Western blotting using a phosphorylation-state specific S40 antibody. Phosphoprotein levels were normalized for the total level of phosphoprotein in the sample as detected by a pan-TH antibody. Integrated changes in phosphorylation state were calculated, relative to control samples, over the 60-min period after drug treatment for each compound. *p < 0.01; ***p < 0.001 compared with control, ^‡ p < 0.001 compared with ITI-007, clozapine, and aripiprazole; ^† p < 0.05 compared with aripiprazole, ANOVA with Newman–Keuls post hoc test