Table 1.

Amino acid contents of high-lysine group (HLG) transgenic lines of rice

	WT (mg/g protein)	% difference from WT
		pA1-High	pA2-High	pA3-High	pA4-High	pB3-High	pB4-High
Asx	90.00 ± 3.63	0.00 ± 1.57	−2.91 ± 1.87	−0.75 ± 1.23	0.02 ± 4.53	−6.47 ± 2.91	−0.64 ± 0.64
Thr	30.13 ± 0.66	3.81 ± 1.42	6.05 ± 0.54	7.77 ± 0.13	2.81 ± 2.60	6.05 ± 2.23	6.12 ± 0.82
Ser	39.10 ± 1.91	5.19 ± 2.71	5.46 ± 3.84	5.33 ± 0.44	0.35 ± 6.83	6.68 ± 3.64	0.23 ± 0.25
Glx	156.12 ± 7.47	−1.72 ± 0.19	−2.25 ± 1.19	−8.49 ± 0.32	−4.13 ± 0.29	−4.62 ± 0.24	−9.18 ± 0.43
Pro	37.96 ± 1.38	−1.29 ± 2.32	−0.49 ± 3.83	5.04 ± 2.30	32.21 ± 2.40	48.52 ± 0.76	29.84 ± 3.08
Gly	42.39 ± 1.01	4.51 ± 0.40	4.32 ± 0.13	11.70 ± 0.29	4.84 ± 0.05	4.03 ± 0.28	2.27 ± 0.22
Ala	47.84 ± 2.28	0.02 ± 0.42	−0.02 ± 0.18	2.93 ± 0.46	0.33 ± 0.13	−1.16 ± 0.30	−0.93 ± 0.15
Val	48.71 ± 1.85	−0.38 ± 0.64	−2.76 ± 0.17	−1.89 ± 0.05	−2.05 ± 0.43	−3.85 ± 0.27	−3.70 ± 0.50
Ile	33.22 ± 1.55	−2.37 ± 0.66	−3.56 ± 0.58	−3.28 ± 0.39	−3.50 ± 0.36	−4.75 ± 0.27	1.26 ± 0.11
Leu	73.21 ± 4.57	−4.31 ± 0.26	−4.82 ± 0.29	−8.99 ± 0.19	−7.00 ± 0.05	−7.53 ± 0.12	−6.70 ± 0.45
Tyr	22.04 ± 2.41	−4.51 ± 0.78	−2.80 ± 1.47	−13.95 ± 0.72	−8.87 ± 0.60	−8.20 ± 0.74	−1.98 ± 0.38
Phe	50.09 ± 2.82	−1.43 ± 0.08	−3.21 ± 0.19	−8.50 ± 0.85	−4.89 ± 0.15	−3.79 ± 0.15	−3.96 ± 0.51
His	24.04 ± 0.51	3.23 ± 0.12	4.97 ± 0.48	11.99 ± 0.92	1.97 ± 0.39	1.66 ± 2.02	−1.63 ± 3.59
Lys	35.46 ± 1.17	9.46 ± 0.11	10.98 ± 0.37	35.65 ± 0.31	10.65 ± 0.07	7.49 ± 1.66	24.39 ± 1.33
Arg	70.99 ± 2.10	−0.31 ± 0.61	5.26 ± 0.95	2.29 ± 0.44	−2.19 ± 1.32	−1.90 ± 0.50	2.25 ± 0.12
Total	801.29

Data were corrected for norleucine as an internal standard in each sample and are mean ± SD from duplicated samples. The amino acid content of wild-type (WT) seeds (in italics) and the percent change in transgenic lines are shown. Data in bold represent the two highest lysine elevations from the pA3 and pB4 constructs, respectively

Asx asparagine and aspartic acid, Glx glutamine and glutamic acid