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. 2015 Jan 22;5:7959. doi: 10.1038/srep07959

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(a) NRP cultures from embryonic zebrafish were cultured for 2 and 7 d, respectively. Representative images show the formation of cell aggregates that became interconnected by neurites. Both the size of the aggregates and the density of neurites extending from the aggregates increased until day 7. Immunocytochemical analyses demonstrated the expression of (b) polysialilated-neural cell adhesion molecule (PSA-NCAM) and (c) neurofilaments (NF) in most of the cells within the aggregates at 2 and 7 d in vitro. (d) Only a few cells showed the expression of glial fibrillary acid proteins (GFAP). Nuclei were stained with DAPI (blue). All scale bars, 50 μm.