Skip to main content
. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Proteomics Clin Appl. 2014 Sep 15;8(0):783–795. doi: 10.1002/prca.201300077

Figure 5. RNA-Seq, Protein Sequence, and LC-MRM Verification of Constant and Variable Region Peptides for H929 MM Cells.

Figure 5

RNA-seq and in silico translation produced a protein sequence (A), which was used to generate tryptic peptides for monitoring (underlined sequences). The peptides from the variable and constant regions are alternately placed in bold font to show their sequences. LC-MRM could detect three constant (C1–C3) and six variable region (V1–V6) peptides from digests of conditioned media (B). Peptide sequences are provided again in the text. Spiked H929 Igκ could also be detected in control serum (C).