Table 3.
Summary of positive infection with Babesia canis based on PCR and IFA tests results
| Group* | Animal ID | PCR | IFA | Blood smear** | Infected*** |
|---|---|---|---|---|---|
| Results for experiment 1 | |||||
| Group 1 Untreated | 1-1-1 | POS | POS | POS | YES |
| 1-1-2 | POS | POS | POS | YES | |
| 1-1-3 | POS | POS | POS | YES | |
| 1-1-4 | POS | POS | POS | YES | |
| 1-1-5 | POS | POS | POS | YES | |
| 1-1-6 | POS | POS | - | YES | |
| 1-1-7 | POS | POS | POS | YES | |
| Infected = 100% | |||||
| Group 2 Treated on day −28 | 1-2-1 | NEG | NEG | - | NO |
| 1-2-2 | POS | POS | POS | YES | |
| 1-2-3 | NEG | NEG | - | NO | |
| 1-2-4 | NEG | NEG | - | NO | |
| 1-2-5 | NEG | NEG | - | NO | |
| 1-2-6 | NEG | NEG | - | NO | |
| 1-2-7 | POS | NEG | - | NO | |
| Infected = 14.3% | |||||
| Group 3 Treated on day −21 | 1-3-1 | NEG | NEG | - | NO |
| 1-3-2 | NEG | NEG | - | NO | |
| 1-3-3 | NEG | NEG | - | NO | |
| 1-3-4 | POS | NEG | - | NO | |
| 1-3-5 | NEG | NEG | - | NO | |
| 1-3-6 | NEG | NEG | - | NO | |
| 1-3-7 | NEG | NEG | - | NO | |
| Infected = 0% | |||||
| Group 4 Treated on day −14 | 1-4-1 | NEG | NEG | - | NO |
| 1-4-2 | NEG | NEG | NEG | NO | |
| 1-4-3 | NEG | NEG | - | NO | |
| 1-4-4 | NEG | NEG | - | NO | |
| 1-4-5 | NEG | NEG | - | NO | |
| 1-4-6 | NEG | NEG | NEG | NO | |
| 1-4-7 | NEG | NEG | - | NO | |
| Infected = 0% | |||||
| Group 5 Treated on day −7 | 1-5-1 | NEG | NEG | - | NO |
| 1-5-2 | NEG | NEG | - | NO | |
| 1-5-3 | NEG | NEG | - | NO | |
| 1-5-4 | NEG | NEG | - | NO | |
| 1-5-5 | POS | NEG | - | NO | |
| 1-5-6 | POS | POS | POS | YES | |
| 1-5-7 | NEG | NEG | - | NO | |
| Infected = 14.3% | |||||
| Results for experiment 2 | |||||
| Group 1 Untreated | 2-1-1 | POS | NEG | POS | NO |
| 2-1-2 | POS | POS | POS | YES | |
| 2-1-3 | POS | NEG | POS | NO | |
| 2-1-4 | POS | POS | POS | YES | |
| 2-1-5 | POS | POS | POS | YES | |
| 2-1-6 | POS | POS | POS | YES | |
| 2-1-7 | POS | POS | POS | YES | |
| 2-1-8 | POS | POS | POS | YES | |
| Infected = 75% | |||||
| Group 2 Treated on day −2 | 2-2-1 | NEG | NEG | NEG | NO |
| 2-2-2 | NEG | NEG | NEG | NO | |
| 2-2-3 | NEG | NEG | NEG | NO | |
| 2-2-4 | NEG | NEG | NEG | NO | |
| 2-2-5 | NEG | NEG | NEG | NO | |
| 2-2-6 | NEG | NEG | NEG | NO | |
| 2-2-7 | NEG | NEG | NEG | NO | |
| 2-2-8 | NEG | NEG | NEG | NO | |
| Infected = 0% | |||||
*Experiment 1: Group 1: untreated dogs ; Group 2: treated on day −28; Group 3: treated on day −21; Group 4: treated on day −14; Group 5: treated on day −7.
Experiment 2: Group 1: untreated dogs ; Group 2: treated on day −2.
All groups were infested with ticks on Day 0.
**Blood smear only prepared if clinical signs observed.
***Dogs declared positive if both IFA and PCR are positive.
POS: dog with a positive IFA or PCR or blood smear analysis.
NEG: dog with a negative IFA or PCR or blood smear analysis.