Table 5.
Single gene effects confirmed for ATPalpha alleles using classical mutants
| Cytological region | Gene | Genotype | Putative function # | ATPα Allele | Nature of interaction | Significance |
|---|---|---|---|---|---|---|
| 10B3 | l(1)10Bb | E04588 | Spliceosome component [44] | CJ10 | Suppressor | * |
| 21B1-21B1 | Galectin | DG25505 | Cell surface protein, galactoside binding [45] | DTS1 | Enhancer | *** |
| 23C9-23C9 | Rbp9 | ∆1 | RNA binding [46] | DTS1 | Enhancer | * |
| 23C9-23C9 | Rbp9 | ∆1 | " | CJ5 | Suppressor | **** |
| 27E-28B1 | Ndae1 | MB05294 | Sodium driven anion exchanger [47] | CJ5 | Suppressor | * |
| 27E-28B1 | Ndae1 | MB05294 | " | DTS1 | Enhancer | * |
| 27E-28B1 | Ndae1 | MB05294 | " | CJ10 | Suppressor | * |
| 29B4-29E4 | Sema-1a | K13702 | Axon guidance signal and receptor [48,49] | DTS1 | Enhancer | * |
| 29B4-29E4 | Glt | EY22126 | Cell surface glycoprotein [50] | DTS1 | Enhancer | **** |
| 29B4-29E4 | Glt | EY22126 | “ | CJ10 | Suppressor | * |
| 30C7-30 F2 | Nckx30C | E00401 | Sodium/Calcium/Potassium exchanger [51] | CJ10 | Enhancer | * |
| 30C7-30 F2 | Ppk11 | MB02012 | Excitatory sodium channel [52] | CJ10 | Suppressor | *** |
| 30C7-30 F2 | Ppk11 | MB02012 | “ | DTS1 | Suppressor | **** |
| 30C7-30 F2 | Ppk11 | MB02012 | “ | CJ5 | Suppressor | **** |
| 30C7-30 F2 | nAChRα6 | MB06675 | ACh receptor subunit | CJ10 | Suppressor | * |
| 30C7-30 F2 | nAChRα6 | MB06675 | “ | CJ5 | Suppressor | **** |
| 31C-32E | FKBP59 | EY03538 | Calcium channel regulator [53] | DTS1 | Enhancer | * |
| 31C-32E | FKBP59 | EY03538 | " | CJ5 | Suppressor | *** |
| 31C-32E | FKBP59 | EY03538 | “ | CJ10 | Suppressor | *** |
| 33A8-33B1 | Pde1c | C04487 | cAMP/cGMP phosphodiesterase [54] | CJ5 | Suppressor | **** |
| 34E4-35B4 | Dyrk2 | 1 | Serine/Threonine kinase [55] | DTS1 | Enhancer | *** |
| 34E4-35B4 | Dyrk2 | 1 | " | CJ10 | Suppressor | **** |
| 34E4-35B4 | Nimb5 | MI01793 | Bacterial defense | CJ10 | Suppressor | ** |
| 34E4-35B4 | nAChRα5 | MB11647 | ACh Receptor subunit | CJ10 | Suppressor | * |
| 35 F1-36A1 | Cact | 7 | Inhibitor of NF-κB [56] | CJ10 | Suppressor | **** |
| 36A8-36 F1 | Beat-Ia & Fas3 | 3/E25 | Neuronal immunoglobulin-like proteins | CJ5 | Suppressor | * |
| 25 F1-36A1 | CG5888 | MB00188 | Toll 3 like receptor | DTS1 | Enhancer | **** |
| 25 F1-36A1 | CG5888 | MB00188 | " | CJ5 | Suppressor | **** |
| 46 F1-47A9 | CG42732 | MB04544 | Predicted potassium channel | DTS1 | Enhancer | **** |
| 46 F1-47A9 | Rpl41/NaCP60E | EP348 | Ribosomal protein; voltage-gated Na+ channel [57] | CJ10 | Suppressor | * |
| 46 F1-47A9 | CG42732 | MB04544 | Predicted potassium channel | CJ5 | Suppressor | ** |
| 46 F1-47A9 | Gαo | MI00833 | Heterotrimeric G-protein subunit | CJ10 | Suppressor | **** |
| 46 F1-47A9 | CYP49A1 & Gαo | MB04922 | Cytochrome P450 & heterotrimeric G-protein subunit | DTS1 | Enhancer | **** |
| 50B1 | CG33156 | MB05931 | Predicted NAD+ kinase | DTS1 | Enhancer | **** |
| 57C5-57 F6 | Pu | r1 | GTP cyclohydrolase [58] | CJ5 | Suppressor | ** |
| 57C5-57 F6 | Pu | r1 | “ | CJ10 | Suppressor | **** |
| 60E6-60E11 | Pain | EP2451 | TRP calcium channel [59] | DTS1 | Enhancer | ** |
| 60E6-60E11 | Pain | EP2451 | “ | CJ10 | Suppressor | **** |
| 60E6-60E11 | Rpl19 | K03704 | Ribosomal component [60] | DTS1 | Enhancer | **** |
| 62E8-63B6 | Spz5 | E03444 | Neurotrophin [61,62] | DTS1 | Enhancer | ** |
| 62E8-63B6 | Spz5 | E03444 | “ | CJ10 | Suppressor | **** |
| 62E8-63B6 | Aly | 1 | Regulator of transcription [63,64] | DTS1 | Enhancer | **** |
| 62E8-63B6 | Rasp | m47 | Palmitoyl transferase [65,66] | DTS1 | Enhancer | ** |
| 62E8-63B6 | Rasp | m47 | “ | CJ10 | Suppressor | **** |
| 63A3-63A3 | Scramb2 | EY01180 | Predicted phosphatidyl serine scramblase | DTS1 | Enhancer | **** |
| 63A3-63A3 | Scramb2 | EY01180 | " | CJ10 | Suppressor | **** |
| 67A2-67D13 | Aay | S042314 | Predicted Phosphoserine phosphatase | CJ10 | Suppressor | ** |
| 93B9-93D4 | Slmb | 295 | Ubiquitin ligase [67,68] | DTS1 | Enhancer | ** |
| 93B9-93D4 | Slmb | 295 | “ | CJ10 | Suppressor | **** |
| 93B9-93D4 | Sec15 | 2 | Protein trafficking [69,70] | DTS1 | Enhancer | ** |
| 93B9-93D4 | Sec15 | 2 | “ | CJ10 | Suppressor | **** |
| 93B9-93D4 | RhoGAP93B | EY07136 | Rac1 GAP [71] | DTS1 | Enhancer | * |
| 98 F10-99B9 | CG14508 | G9163 | Predicted cytochrome C | DTS1 | Enhancer | *** |
| 98 F10-99B9 | CG14508 | G9163 | Predicted cytochrome C | CJ10 | Suppressor | **** |
| 99E1-3Rt | Sro | 1 | Ecdysone biosynthetic pathway | CJ10 | Suppressor | * |
Many genes had an interaction with more than one allele, although some appear to be allele specific. Double mutants were compared to ATPalpha * /+ and heterozygous classical mutant controls. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
#Function per flybase.org and / or listed citation.