Table 3.

Number of knockouts for the biomass reaction in selected networks

Model		Single knockouts		Double knockouts
reaction id	cl. size	classes	reactions	cl. pairs	reac. pairs
E. coli iJO1366
Ec_biomass_iJO1366_WT_53p95M	20	101	343	130	339
Ec_biomass_iJO1366_core_53p95M	1	80	288	90	268
S. cerevisiae iND750
biomass_SC4_bal	26	54	156	60	142
M. tuberculosis iNJ661
biomass_Mtb_9_60atp	160	64	154	48	83
S. aureus iSB619
SA_biomass_1a	8	25	63	59	157
SA_biomass_5a	1	58	215	54	100
H. pylori iIT341
BiomassHP_published	189	36	76	41	81

class size: Number of reactions in the same coupling class as the biomass reaction, i.e., number of reactions that carry flux if and only if the biomass reaction carries flux.

Single Knockouts: Number of different single knockouts (classes and reactions) that block the biomass reaction. Only reactions that are not partially coupled to the biomass (from a different reaction class) are counted.

Double Knockouts: Number of different double knockouts (class pairs and reaction pairs) that block the biomass reaction when combined. Only reactions that are not directionally coupled to the biomass are counted.