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. 2014 Dec 30;14:354. doi: 10.1186/s12870-014-0354-8

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© Abarca et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Experimental system used for analysis. A, B) Embryogenic masses of Pinus radiata after 7 (P7) and 14 (P14) days of proliferation. Embryogenic tissue (in red) was stained with 1% acetocarmine. Bar: 2 mm. C) Early-maturation embryo at polarization stage (M1). Bar: 0.5 mm. D) Late-maturation embryo at tissue differentiation stage (M3). Bar: 0.8 mm. E) Hypocotyls from 21-day-old seedlings treated with 10 μM indole-3-butyric acid (IBA) after 28 days of culture. F, G) Hypocotyls (F) and epicotyls (G) from 90-day-old seedlings treated with 10 μM IBA.