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. 2014 Dec 21;14:330. doi: 10.1186/s12866-014-0330-3

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© Ishida et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of genes encoding glycolytic enzymes including aldolase A, GAPDH and enolase, and the kinetic changes in HEp-2 cells with chlamydial infection. (A) Representative RT-PCR images showing expression of genes encoding aldolase A, chlamydial 16S rRNA and β-actin in infected HEp-2 cells. C. pneumoniae infected cells at MOI 5 were collected at 24, 48 and 60 h. uninf, uninfected HEp-2 cells. (B) Kinetic changes in glycolytic enzyme gene expression in HEp-2 cells with chlamydial infection. The gene expression changes were evaluated by qRT-PCR. The values were normalized to that of β-actin. Data show mean ± SD from at least three independent experiments. N.S., not significant.