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. 2015 Jan 16;15:1. doi: 10.1186/s12861-015-0051-3

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© Krüger and Bohrmann; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characteristic patterns of intracellular pH (pH _i ). CFDA staining, SIM, S9 to S10B, A, B, C: representative grey-scale images, A´, B´, C´: corresponding pseudocolour images. Alkaline pH_i is indicated by strong, acidic pH_i by weak fluorescence intensity (see scale bar). D: schematic drawings of characteristic pH_i-patterns; alkaline regions are shown in blue (staining in Ooc is not considered). Between S9 and S10B, an anteroposterior pH_i-gradient develops within the NC group, the NC adjacent to the anterodorsal region of the Ooc (asterisk) being most acidic. Usually, an anteroposterior pH_i-gradient was observed within the FC epithelium, the cFC being most acidic. Also the migrating BC are characterized through an acidic pH_i. Near the posterior oolemma (Ool), the Ooc is alkaline compared to the remaining regions. For abbreviations, see Figure 1.