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. 2015 Jan 14;12:5. doi: 10.1186/s12974-014-0228-x

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© Viceconte et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Flow-cytometric analysis of BV2 cell viability. Cells were analyzed using propidium iodide and annexin V. (A) Control cells. (B) Staurosporine (1 μM for 12 h, as death control). (C) BV2 cells + 1 μg/ml of neuromelanin for 24 h. (D) Quantification of cell survival. Results are mean ± standard deviation of three independent experiments, and are expressed as percentage of control values. Statistical analysis was made using Student’s t test. *, P < 0.01 compared with control values. Cont., control; NM, neuromelanin; PI, propidium iodide; STS, staurosporine.