Fig. 1.

CII induces ACAID in C57BL6 and DBA/1 mice via AC injection as confirmed by DTH assays (A) CII induces ACAID in C57BL6 mice via AC injection. (B) CII induces ACAID in DBA/1 mice via AC injection. A DTH assay was performed as described in the materials and methods section. To induce ACAID, mice received an AC injection of CII followed by subcutaneous immunization with CII/CFA on day 7. On day 14, mice were challenged with CII (500 μg in 20 μl) intradermally in the left ear pinna, and 20 μl 10 mM acetic acid alone was injected into the right ear pinna as an internal control. Induction of ACAID was confirmed by inhibition of ear swelling responses after 24 and 48 h. The positive control mice were subcutaneously immunized with CII/CFA on day 7 and with CII on day 14, whereas the negative control mice only received the day 14 intradermal injection of CII. P values <0.05 were considered to be significant (*). CII, type II collagen; ACAID, anterior chamber-associated immune deviation; AC, anterior chamber; DTH, delayed-type hypersensitivity; CFA, complete Freund's adjuvant.