Fig. 2.
In vitro-generated CII-specific ACAID APCs induce ACAID in C57BL6 and DBA/1 mice as confirmed by DTH assays. (A) In vitro-generated CII-specific ACAID APCs inhibit DTH responses in C57BL6 mice. CII-specific ACAID APCs were generated in vitro, as described, and were adoptively transferred into C57BL6 mice (1 × 106 cells/mouse). A DTH assay was performed, as described. (B) In vitro-generated CII-specific ACAID APCs inhibit DTH responses in DBA/1 mice. CII-specific ACAID APCs were generated in vitro, as described, and were adoptively transferred into DBA/1 mice (1 × 106 cells/mouse). A DTH assay was performed, as described. Induction of ACAID was confirmed by inhibition of ear swelling responses after 24 and 48 h. P values <0.05 were considered to be significant (*). CII, type II collagen; ACAID, anterior chamber-associated immune deviation; DTH, delayed-type hypersensitivity; APC, antigen-presenting cell.