Figure 4.

Comparison of cisplatin sensitivity and the presence of sphere-forming cells in surgical samples of NSCLC patients. Surgical samples of NSCLC patients that had not received any pharmacological treatment were disaggregated. Half of the cells obtained were used to analyze cisplatin sensitivity and their IC50 determined. The other half was cultured in defined media under nonadherent conditions and the presence of cell spheres determined after 30 days in culture. The IC50 values obtained are represented for each patient. Patient samples that contained sphere-forming cells are indicated with open circles (CSC) and those that did not with close circles (NO CSC). A IC50 value of 2 μg/mL was considered the limit between cisplatin sensitive and resistant cells. Bar, median value of each group.