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. 2014 Jun 28;3(5):1112–1125. doi: 10.1002/cam4.277

Figure 3.

Figure 3

OPG knockdown by siRNA reduces metastasis of MDA-MB-231 cells in the chick embryo spontaneous metastasis model. (A) MDA-MB-231 cells were transfected with two separate OPG siRNAs or with negative control siRNA. OPG expression was measured by ELISA in cell culture supernatants after 24-, 48-, and 72-h incubation (*P < 0.05 significantly different from siRNA control cells, n ≥ 3). OPG siRNA #2 or negative control siRNA cells were incubated 1 week in the chick embryo metastasis model and (B) primary tumors were collected and weighed and (C) chick lower CAM and liver analyzed by quantitative PCR for human Alu DNA (*P < 0.05, n = 17). All data are represented as mean ± SEM.