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. 2014 Aug 13;3(5):1211–1224. doi: 10.1002/cam4.298

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© 2014 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Oct4 and Klf4 are acetylated by p300 in vivo. (A) Whole cell lysates (WCLs) of 293T cells transfected with HA-tagged p300 or CBP with indicated Flag-tagged constructs were subjected to immunoprecipitation (IP) with Flag antibody. The Flag-IPs and WCLs were immunoblotted with indicated antibodies. Acetylation was detected by a Lys-acetylation (Ac-K) antibody. (B) WCLs of 293T cells transfected with indicated constructs were subjected to immunoprecipitation with Flag antibody. The Flag-IPs and WCLs were immunoblotted with indicated antibodies. (C) WCLs of 293T cells transfected with indicated constructs were subjected to immunoprecipitation with HA antibody. The HA-IPs and WCLs were immunoblotted with Flag and HA antibodies.