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. 2014 Aug 13;3(5):1211–1224. doi: 10.1002/cam4.298

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© 2014 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Mapping of Sox2 acetylation sites in vivo. (A) Schematic illustration of a series of generated Sox2 truncation mutations. (B) WCLs of 293T cells transfected with various Sox2 mutants were subjected to immunoprecipitation with Flag antibody. The Flag-IPs and WCLs were immunoblotted with Ac-K and Flag antibodies. (C) WCLs of 293T cells transfected with various Sox2 K-to-R substitution mutants were subjected to immunoprecipitation with Flag antibody. The Flag-IPs and WCLs were immunoblotted with Ac-K and Flag antibodies. (D) WCLs of 293T cells transfected with HA-p300 and various Sox2 truncation mutants were subjected to immunoprecipitation with Flag antibody. The Flag-IPs and WCLs were immunoblotted with Ac-K and Flag antibodies. WCLs, whole cell lysates; IP, immunoprecipitation.