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. 2014 Aug 13;3(5):1211–1224. doi: 10.1002/cam4.298

Figure 4.

Figure 4

Mapping of Oct4 acetylation sites in vivo. (A) Schematic illustration of a series of generated Oct4 truncation mutations. (B and C) WCLs of 293T cells transfected with HA-p300 and various Oct4 truncation mutants were subjected to immunoprecipitation with Flag antibody. The Flag-IPs and WCLs were immunoblotted with indicated antibodies. (D) WCLs of 293T cells transfected with HA-p300 and various Oct4 K-to-R substitution mutants were subjected to immunoprecipitation with Flag antibody. The Flag-IPs and WCLs were immunoblotted with indicated antibodies. WCLs, whole cell lysates; IP, immunoprecipitation.