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. 2014 Aug 13;3(5):1211–1224. doi: 10.1002/cam4.298

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© 2014 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Mapping of Klf4 acetylation sites in vivo. (A) Schematic illustration of a series of Klf4 truncation mutations generated. (B) WCLs of 293T cells transfected with HA-p300 and various Klf4 truncation mutants were subjected to immunoprecipitation with Flag antibody. The Flag-IPs and WCLs were immunoblotted with indicated antibodies. (C and D) WCLs of 293T cells transfected with HA-p300 and various Klf4 truncation or K-to-R substitution mutants were subjected to immunoprecipitation with Flag antibody. The Flag-IPs and WCLs were immunoblotted with indicated antibodies. WCLs, whole cell lysates; IP, immunoprecipitation.