Table 2.

Primers used for vector-borne pathogens detection by PCR in the study

Gene target	PCR target	Name	Primer sequence	Fragment length	Reference
Mitosin gene	Canis familiaris	CAN-F	5′-CTTGTCACGGTAAGGTTC-3′	290-bp	[14]
Mitosin gene	Canis familiaris	CAN-R	5′-CTGATGTATTTCCTGCACCAAG-3′	290-bp	[14]
Vir-B9 protein gene	Ehrlichia canis	Ehr1401F	5′-CCATAAGCATAGCTGATAACCCTGTTACAA-3′	380-bp	[15]
Vir-B9 protein gene	Ehrlichia canis	Ehr1780R	5′-TGGATAATAAAACCGTACTATGTATGCTAG-3′	380-bp	[15]
GroEL gene	Anaplasma platys	GroAplatys-35 s	5′-AGCGTAGTCCGATTCTCCAGTTTT-3′	515-bp	[16]
GroEL gene	Anaplasma platys	GroAplatys-550as	5′-TCGCCGTTAGCAGAGATGGTAG-3′	515-bp	[16]
AnkA gene	Anaplasma phagocytophilum	AnkAP-2074s	5′-GGCAAATGAGGCAAGTAACC-3′	741-bp	[16]
AnkA gene	Anaplasma phagocytophilum	AnkAP-2815as	5′-GCCACTACCCAAGGATGATAG-3′	741-bp	[16]
18S rDNA	Babesia spp	Ba103F	5′-CCAATCCTGACACAGGGAGGTAGTGACA-3′	612-bp	[15]
18S rDNA	Babesia spp	Ba721R	5′-CCCCAGAACCCAAAGACTTTGATTTCTCTCAAG-3′	612-bp	[15]
18S rDNA	Hepatozoon canis	Hep F	5′-ATACATGAGCAAAATCTCAAC-3′	666-bp	[17]
18S rDNA	Hepatozoon canis	Hep R	5′-CTTATTATTCCATGCTGCAG-3′	666-bp	[17]
18S rDNA	Leishmania spp	R221	5′-GGTTCCTTTCCTGATTTACG-3′	603-bp	[18]
18S rDNA	Leishmania spp	R332	5′-GGCCGGTAAAGGCCGAATAG-3′	603-bp	[18]