Table 2.
Wheat transporters and their expression during senescence
| Transporter | WT counts* | Differential expression** | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Rice | Wheat | Chromosome | IWGSC ID | HD | 12D | 22D | Senescence | gpc-A1 | gpc-A1/B2 |
| Chloroplastic transporters | |||||||||
| OsFRO1 | TaFRO1 | 2AL | Traes_2AL_2A274FDB8 | 40,148 | 22,892 | 16,515 | |||
| 2BL | Traes_2BL_C7CDCB39A | 48,913 | 30,272 | 13,838 | ⬇ | ||||
| OsFRO2 | TaFRO2 | 2AL | Traes_2AL_7E818894E | 3 | 1 | 6 | |||
| 2BL | Traes_2BL_E11AA2D03 | 49 | 8 | 9 | |||||
| OsHMA1 | TaHMA1 | 7AL | Traes_7AL_84D5BAE85 | 1,143 | 1,256 | 1,249 | |||
| 7BL | Traes_7BL_041308E74 | 674 | 614 | 600 | |||||
| TaHMA1-like | 5AL | Traes_5AL_C89EEBE50 | 1 | 2 | 1 | ||||
| 5BL‡ | Traes_5BL_F83C809F0 | 74 | 78 | 116 | |||||
| OsHMA2 | TaHMA2 | 7AL | Traes_7AL_8304348B7 | 125 | 328 | 1,079 | ⬆ | ||
| 7BL | Traes_7BL_C46BC291C/ Traes_7BL_8C24C1025 | 185 | 356 | 1,205 | ⬆ | ||||
| TaHMA2-like | 7AL | Traes_7AL_6AE850114 | 13 | 75 | 188 | ⬆ | ⬆ | ⬆ | |
| 7BL | Traes_7BL_0CF58CF4E | 2 | 13 | 17 | ⬆ | ||||
| OsHMA3 | TaHMA3 | 5BL | Traes_5BL_D6C3DC326 | - | - | - | |||
| Vacuolar transporters | |||||||||
| OsVIT1 | TaVIT1 | 2AL | Traes_2AL_A3A25F40E | 59 | 44 | 36 | |||
| 2BL | Traes_2BL_54954138A | 74 | 61 | 54 | |||||
| OsVIT2 | TaVIT2 | 5BL | Traes_5BL_7CE3EDE29 | 1,938 | 566 | 450 | |||
| OsNRAMP1 | TaNRAMP1 | 7AL | Traes_7AL_76159C6DA | - | - | - | |||
| 7BL | Traes_7BL_03741F576 | 3 | 6 | 1 | |||||
| OsNRAMP2 | TaNRAMP2 | 4AS | Traes_4AS_BBF51CA2E | 437 | 927 | 3,607 | ⬆ | ||
| 4BL | Traes_4BL_C6A3F5C8A | 637 | 743 | 2,252 | |||||
| OsNRAMP3 | TaNRAMP3 | 7AL | Traes_7AL_08B2A7BB2 | 640 | 704 | 469 | |||
| 7BL | Traes_7BL_CA6B7C9E6 | 551 | 571 | 352 | |||||
| OsNRAMP4 | TaNRAMP4 | 6AS | Traes_6AS_B9B4AD633 | 1 | 1 | 1 | |||
| 6BS | N/A | 3 | 5 | 5 | |||||
| OsNRAMP5 | TaNRAMP5 | 4AS | Traes_4AS_5D4904831 | 6 | 2 | 2 | |||
| 4BL | Traes_4BL_04B01EA0C/ Traes_4BL_CFD804098 | 4 | 2 | 2 | |||||
| None | TaNRAMP6 | 3AS | Traes_3AS_538630B00 | - | - | - | |||
| 3B | Traes_3B_73F0469A5 | 10 | 9 | 19 | |||||
| OsNRAMP7 | TaNRAMP7 | 5AS | Traes_5AS_213BE4D84 | 215 | 176 | 151 | |||
| 5BS | Traes_5BS_DE1CD2DA4 | 127 | 88 | 103 | |||||
| None | TaNRAMP8 | 4AL | Traes_4AL_2E796609C | - | - | - | |||
| 4BS | Traes_4BS_9337E9B2F | - | - | - | |||||
| OsZIFL1 | TaZIFL1 | 3AS | Traes_3AS_C25151458 | 378 | 73 | 19 | ⬇ | ||
| 3B | Traes_3B_BD45F6269/ Traes_3B_45F864939 | 249 | 45 | 5 | ⬇ | ||||
| OsZIFL1 | TaZIFL1-like1 | 3AS | Traes_3AS_02DE247DA | 125 | 136 | 84 | |||
| 3B | Traes_3B_92383792E/ Traes_3B_B76607C0E | 70 | 76 | 59 | |||||
| OsZIFL2 | TaZIFL2 | 5BL | Traes_5BL_A0B9DE62E | 1 | 2 | 2 | |||
| OsZIFL2 | TaZIFL2-like1 | 3AS | Traes_3AS_E59FB52EC | 8 | 31 | 32 | ⬆ | ||
| 3B | Traes_3B_EDFDD5A12 | 167 | 305 | 383 | |||||
| None | TaZIFL3 | 4AL | Traes_4AL_4231650FC/ Traes_4AL_470869233 | 183 | 218 | 171 | |||
| 4BS | Traes_4BS_1DCF82CB7 | 525 | 1,019 | 743 | ⬆ | ||||
| None | TaZIFL7 | 5AL | Traes_5AL_37BFFFD9E | 397 | 392 | 249 | ⬆ | ⬆ | |
| 5BL | Traes_5BL_6E4AE0146 | 130 | 108 | 48 | |||||
| None | TaZIFL8 | 4AL | Traes_4AL_5C7A4DA54 | 21 | 19 | 13 | |||
| 4BS | Traes_4BS_44732F50F | 141 | 112 | 160 | |||||
| None | TaZIFL9 | 5AL | Traes_5AL_0599F7BC5 | - | - | - | |||
| 5BL | N/A | - | - | - | |||||
| Plasma membrane transporters | |||||||||
| OsYSL1 | TaYSL1 | 3AS | Traes_3AS_FB4110335 | 10 | 7 | 8 | |||
| 3B | Traes_3B_17BC3E1E2 | 1 | 1 | 1 | |||||
| OsYSL2 | TaYSL2 | 6AL | Traes_6AL_850660AC3 | 56 | 35 | 53 | |||
| 6BL | Traes_6BL_3DD0BA741 | 51 | 61 | 62 | |||||
| OsYSL6 | TaYSL6 | 2AL | Traes_2AL_7B0F93F84 | 364 | 526 | 986 | ⬆ | ||
| 2BL | Traes_2BL_0CBCC13AD | 68 | 101 | 203 | |||||
| OsYSL9 | TaYSL9 | 2AL | Traes_2AL_CFCA01C76 | 725 | 995 | 1,517 | ⬆ | ||
| 2BL | N/A | 15 | 18 | 28 | |||||
| OsYSL10 | TaYSL10 | 6AL | Traes_6AL_5642D5B44 | 42 | 46 | 35 | |||
| OsYSL11 | TaYSL11 | 2AL | Traes_2AL_377C8CDEA | 0 | 0 | 0 | |||
| 2BL | Traes_2BL_68E0CA743 | 3 | 5 | 3 | |||||
| OsYSL12 | TaYSL12 | 2AL‡ | Traes_2AL_CC6133527 | 554 | 701 | 1,389 | |||
| 2BL | Traes_2BL_2BE05F104 | 437 | 563 | 454 | ⬇ | ||||
| OsYSL13 | TaYSL13 | 2AL | Traes_2AL_F707FF2C3.3 | 14 | 20 | 75 | |||
| 2BL | Traes_2BL_A14EA5AE4 | 1 | 1 | 3 | |||||
| TaYSL13-like | 2BL | Traes_2BL_A14EA5AE4 | 7 | 19 | 85 | ||||
| OsYSL14 | TaYSL14 | 6AL | Traes_6AL_7FB45D4DE | 814 | 894 | 733 | |||
| 6BL | Traes_6BL_7FFC46B84 | 500 | 628 | 493 | |||||
| OsYSL15 | TaYSL15 | 6AL | Traes_6AL_E36FCEF64 | 523 | 486 | 969 | |||
| 6BL | Traes_6BL_D65EC1432 | 88 | 95 | 199 | |||||
| TaYSL15-like | 1AL | Traes_1AL_C6A0E255E | 4 | 7 | 9 | ||||
| 1BL | Traes_1BL_CA93E6359 | 2 | 2 | 2 | |||||
| OsYSL16 | TaYSL16 | 2BL | Traes_2BL_4A1181B731 | 2,736 | 2,361 | 2,011 | |||
| None | TaYSL18 | 2AL | Traes_2AL_2F91AF932 | 225 | 144 | 84 | ⬇ | ||
| 2BL | Traes_2BL_6C5206B6D | 531 | 499 | 510 | |||||
| OsIRT1 | TaIRT-like1 | 4AL | Traes_4AL_9D79BE8FB | 5 | 7 | 10 | |||
| 4BS | Traes_4BS_6527BBD54 | 8 | 6 | 7 | |||||
| OsIRT2 | TaIRT-like 2 | 4AL | Traes_4AL_9F6B106F3 | 9 | 21 | 30 | ⬆ | ||
| OsZIP10 | TaZIP10 | 7AL | Traes_7AL_A13A246B4 | 333 | 596 | 531 | ⬆ | ⬆ | |
| 7BL | Traes_7BL_E5CFC3DCE/ Traes_7BL_5C965DB64 | 267 | 531 | 482 | ⬆ | ⬆ | |||
| OsZIP10 | TaZIP10-like1 | 7AL | Traes_7AL_F1D611563/ Traes_7AL_893DEB3EB | 7 | 229 | 279 | ⬆ | ⬆ | ⬆ |
| 7BL | Traes_7BL_12C63350C | 5 | 654 | 647 | ⬆ | ⬆ | ⬆ | ||
| OsZIP8 | TaZIP13-like1 | 6BS | Traes_6BS_7D630200B | 2 | 2 | 2 | |||
| TaZIP13-like2 | 2AS | Traes_2AS_4CA7607E5 | 277 | 4,469 | 3,207 | ⬆ | ⬆ | ⬆ | |
| 2BS | Traes_2BS_9D8F265EC | 79 | 916 | 787 | ⬆ | ⬆ | ⬆ | ||
| TaZIP13-like3 | 2AL | Traes_2AL_BE05B34FF | 125 | 129 | 135 | ||||
| 2BL | Traes_2BL_A1BCBD2BE | 102 | 1,613 | 1,466 | ⬆ | ⬆ | ⬆ | ||
| OsZIP1 | TaZIP1† | 3AL | Traes_3AL_DC3D5F65E | 2 | 1 | 4 | |||
| 3B | Traes_3B_3C18D89F8 | 3 | 4 | 8 | |||||
| OsZIP2 | TaZIP2† | 5BL | N/A | 17 | 9 | 9 | |||
| OsZIP2 | TaZIP2-like | 6AS | Traes_6AS_75C6428051 | - | - | - | |||
| 6BS | N/A | 73 | 65 | 34 | |||||
| OsZIP3 | TaZIP3† | 2AL | Traes_2AL_3983FD077 | 21 | 115 | 138 | ⬆ | ⬆ | ⬆ |
| 2BL | Traes_2BL_23F1C8743 | 61 | 130 | 291 | ⬆ | ⬆ | |||
| OsZIP5 | TaZIP5 | 4AS | Traes_4AS_F5F7D2A8D | 0 | 1 | 3 | |||
| 4BL | Traes_4BL_68691F1FC | 10 | 76 | 176 | ⬆ | ⬆ | |||
| OsZIP6 | TaZIP6† | 1AS | Traes_1AS_A6EF18CC1 | 527 | 488 | 439 | |||
| 1BS | Traes_1BS_B734EDEA7 | 413 | 532 | 478 | |||||
| OsZIP7 | TaZIP7† | 1AS | Traes_1AS_EC8891094/ Traes_1AS_50323685E | 216 | 606 | 880 | ⬆ | ⬆ | ⬆ |
| 1BS | Traes_1BS_D68F0BED6 | 317 | 1,194 | 1,566 | ⬆ | ⬆ | ⬆ | ||
| OsZIP11 | TaZIP11 | 1AS | Traes_1AS_7DC2CB902 | 378 | 357 | 519 | |||
| 1BS | N/A | 193 | 186 | 281 | |||||
| OsZIP13 | TaZIP15 | 6AS | Traes_6AS_4E1D574BC | 322 | 354 | 332 | |||
| 6BS | Traes_6BS_147BF2D07 | 726 | 678 | 513 | |||||
| OsZIP14 | TaZIP14† | 3AS | Traes_3AS_F46E02204/ Traes_3AS_15A221AD0 | 97 | 77 | 103 | |||
| 3B | Traes_3B_B7D3B69FD | 229 | 192 | 213 | |||||
| OsZIP16 | TaZIP16 | 7AL | Traes_7AL_DFE86911E | 15 | 14 | 9 | |||
| 7BL | Traes_7BL_2637B2942/ Traes_7BL_EB231D8FF | 12 | 13 | 6 | |||||
| PS biosynthesis genes | |||||||||
| OsNAS1 | TaNAS1 | 2AS | Traes_2AS_D50EEDA84 | 2 | 5 | 4 | |||
| 2BS | N/A | 121 | 117 | 117 | |||||
| OsNAS3 | TaNAS3 | 2AS | Traes_2AS_DEDC612AE/ Traes_2AS_452FED53F | 2,056 | 2,694 | 4,495 | ⬆ | ||
| 2BS | Traes_2BS_CB79BAFB1 | 5,362 | 5,109 | 7,789 | ⬆ | ||||
| OsNAAT1 | TaNAAT1 | 1AL | Traes_1AL_9D6B86169 | 47 | 88 | 102 | ⬆ | ⬆ | |
| OsNAAT1 | TaNAAT2 | 1AL | Traes_1AL_BCD7C5B8B | 127 | 280 | 269 | ⬆ | ⬆ | ⬆ |
| 1BL | Traes_1BL_D8276D3DB | 188 | 453 | 602 | ⬆ | ⬆ | |||
| OsDMAS1 | TaDMAS1 | 4AS | Traes_4AS_887399584 | 27 | 26 | 17 | |||
| 4BL | N/A | 1222 | 883 | 1134 | |||||
† Source: Tiong et al. [21]. All NRAMP genes from Borrill et al. [7]. TaDMAS from Bashir et al. [26]. ‡ Predicted non-functional protein.
*Counts are normalized values of reads uniquely mapped to the genomic loci corresponding to each wheat transporter gene. **Arrows indicate whether the gene was significantly upregulated (⬆) or downregulated (⬇) during senescence in WT plants or in WT vs. gpc-A1 or WT vs. gpc-A1/gpc-B2 comparisons (⬆ = significantly higher in WT).