| Buffer D (0.1 M) | 5 µL |
| pGEM3 Carrier DNA (100 µg/mL) | 200 ng |
| MgCl2 (0.1 M) | 3 µL |
| Acetyl-Coenzyme A (1 mM) | 2 µL |
| HeLa Nuclear Extract | 15 µL |
| ddH2O | to 30 µL |
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