| ddH2O | 8.5 µL |
| pGEM3 Carrier DNA (100 µg/mL) | 2 µL |
| NP-40 (1%) | 0.5 µL |
| MgCl2 (0.1 M) | 3 µL |
| BSA (10 µg/uL) | 1 µL |
| Buffer D (0.1 M) | 24 µL |
| DNA-Bead slurry (50ng/uL) | 1 µL |
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