| Primer for primer extension (10 µM) | 10 pmol |
| T4 PNK (10 units/µL) | 1 µL |
| PNK reaction buffer (10×) | 5 µL |
| gamma ATP(P32) (>5000 Ci/mmol) | 10 µL |
| DTT (100 mM) | 0.5 µL |
| ddH2O | to 50 µL |
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