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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: J Immunol. 2014 Aug 8;193(6):2821–2830. doi: 10.4049/jimmunol.1302498

FIGURE 2.

FIGURE 2

LPS-mediated death in GM-DC cultures is dependent on NOS2 expression and NO production. (A) WT GM-DCs were either left unstimulated or activated with LPS in the presence or absence of the iNOS inhibitor SEITU and monitored daily for viability by FACS analysis of 7-AAD+ cells. (B) NOS2−/− GM-DCs were either left unstimulated or activated with LPS and monitored daily for viability by FACS analysis of 7-AAD+ cells. (C) Nitrite levels were measured by Griess reaction during 72 h in unstimulated or LPS-activated GM-DCs. (D) GM-DCs were either left unstimulated or activated with LPS, and the iNOS inhibitor SEITU was added to cultures at the time of activation or 1, 2, or 3 d after activation. Cell viability of GM-DCs at 4 d after GM-DC activation by LPS was measured by FACS analysis of 7-AAD+ cells. All graphs in this figure represent mean values ± SD of at least three independent experiments. *p < 0.05.