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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Arthritis Rheumatol. 2014 Mar;66(3):610–621. doi: 10.1002/art.38237

Figure 1. Immunization with 4NPAmC5a mutants.

Figure 1

(A) Model of mC5a based on the crystal structure data of human C5a (3hqa.pdb). Amino acid side chains chosen for 4NPA incorporation are highlighted. (B) SimlyBlue stained SDS PAGE of Ni-NTA purified C5a-4NPA variants (9.9 kDa). Site-specific incorporation of 4NPA was accomplished by mutating the codons for Y17, V21, Y27 or Y35 to the TAG amber codon. The different variants of mC5a were expressed in E. coli [+/− IPTG for wt and +/− 4NPA for mutants (+IPTG)] in presence of the corresponding tRNA/aminoacyl-tRNA-synthetase pair.