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. Author manuscript; available in PMC: 2015 Jul 21.
Published in final edited form as: Nat Commun. 2015 Jan 21;6:6131. doi: 10.1038/ncomms7131

Figure 5. Role of T cell IL-6 response in colitis development in Rag1−/− and IL-10RαMdelRag1−/− mice.

Figure 5

Colitis was induced in Rag1−/− and IL-10RαMdelRag1−/− mice by the transfer of 5×105 C57BL/6 or IL-6Rα−/− CD4+CD25−CD45RBhi T cells. Mice were sacrificed after 8 wk. (A) Representative weight curves, mean ±1 s.e.m., are plotted. *, WT→Rag1−/− vs IL-6Rα−/− →Rag1−/−; △, WT→Rag1−/− vs WT→IL-10RαMdelRag1−/−; □, IL-6Rα−/− →Rag1−/− vs IL-6Rα−/− →IL-10RαMdelRag1−/−; p<0.05, p<0.01, and p<0.001 for 1, 2, and 3 symbols (by ANOVA). No significant differences were observed for WT→IL-10RαMdelRag1−/− vs IL-6Rα−/− →IL-10RαMdelRag1−/−. (B) Histologic scores for colons analyzed at 8 wk. (C) Percent and absolute number of IL-17+ cells among CD4+ T cells in the spleen, MLN and colon. (D) Absolute numbers of CD4+ T cells. (E) Percent and absolute number of IFN-γ+ cells among CD4+ T cells. (F) Percent and absolute number of Foxp3+ Treg cells among CD4+ T cells. *, p<0.05; **, p<0.01; ***, p<0.001 (by t-test). Data are representative of three independent experiments, n=8 per cohort.