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Recombineering Reagents
| Strains | Genotype |
| DH10B | F−mcrA Δ(mrr–hsdRMS–mcrBC) ∅80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu)7649 ga/U ga/K rspL nupG |
| DY380 | DH10B [λcl857 (cro-bioA < > tet] |
| EL250 | DH10B [λcl857 (cro-bioA < > araC-PBADflpe] |
| EL350 | DH10B [λcl857 (cro-bioA < > araC-PBADcre] |
| Selection cassettes | |
| PL451 | FRT–PGK–EM7–NeobpA–FRT–loxP |
| PL452 | loxP–PGK–EM7–NeobpA–loxP |
| Other plasmids | |
| pSK+ | pBluescript |
| PL253 | Modified MC1TK |
