Figure 2.

Schemes for in vitro enzymatic conversions described in this study. (A) A one-pot synthesis of UDP-[¹³C,¹⁵N]GlcNAc utilizes enzymes from bacterial pathways and [¹³C]glucose. Carbohydrate remodeling started with Fc bearing either a mannose-type (B) or a complex-type (C) N-glycan. [¹³C,¹⁵N]GlcNAc is shown as a blue square with a white star in the cartoon figures and by “*N” in the glycan name; residue numbers corresponding to the convention introduced in Figure 1 are given in parentheses.