Table 1. Comparison of the photophysical properties of SdB and BTE a .
Absorption |
Emission |
Brightness | |||
λ max (nm) | ε (M–1 cm–1) | λ em (nm) | Φ b (%) | Φ × ε | |
SdB | 387 | 8800 ± 100 | 478 | 8.3 ± 0.3 | 730 |
BTE | 356 | 4800 ± 100 | 484 | 62 ± 2 | 3000 |
aSpectroscopic measurements were performed at least in triplicate in 100 mM KCl and 50 mM PIPES buffer at pH 7.4 at 25.0 °C.
bQuantum yields are referenced to 1 μM fluorescein (Φ = 0.95 in 0.1 M NaOH).