Table 1.
Deletion Phenotype of S. pombe Genes and Their Closest Homolog in S. cerevisiae
| Gene name | Class | Deletion | Deletion of closest S. cerevisiae homolog17 | |
| SPBC1271.15c | Ia | viable | viable | YOL023w/FM1 |
| SPBC1271.14 | II | viable | viable | YMR062c/ECM40 |
| SPBC1271.13 | Ia | lethal | viable | YJL063c/MRPL8 |
| SPBC1271.12 | Ib | viable | viable | YPLI45c/KES1 |
| SPBC1271.11 | Ib | viable | viable | YNL003c/PET8 |
| SPBC1271.10c | III | viable | viable | YNR055c/HOL1 |
| SPBC1271.09 | Ia | viable | viable | YCR098c/GIT1 |
| SPBC1271.08c | V | viable | — | |
| SPBC1271.07c | IV | viable | — | |
| SPBC1271.06c | V | viable | — | |
| SPBC1271.05c | III | viable | viable | YOR052c |
| SPBC1271.04c | Ia | no deletion | lethal | YHR068w/DYS1 |
| SPBC1271.03c | V | viable | — | |
| SPBC1271.02/stt3 | Ic | lethal | lethal | YGL022w/STT3 |
| SPBC1271.01c | V | viable | — | |
| SPBC106.02c | Ib | no deletion | viable | YKL086w |
| SPBC106.03 | V | viable | — | |
| SPBC106.04 | Ib | viable | viable | YML035c/AMD1 |
| SPBC106.05c | V | viable | — | |
| SPBC106.06 | Id | lethal | lethal | YDL143w/CCT4 |
| SPBC106.07c | Ic | viable | lethal | YGR147c |
| SPBC106.08c | V | viable | — | |
| SPBC106.09/cut4 | Id | lethal2 | lethal | YNL172w/APC1 |
| SPBC106.10/pka1 | Ic | viable3 | viable | YPL203w/TPK2 |
| SPBC106.11c | IV | no deletion | — | |
| SPBC106.12c | IV | no deletion | — | |
| SPBC106.13 | Ib | no deletion | viable | YIL097w |
| SPBC106.14c | Ib | no deletion | lethal | YGR245c/SDA1 |
| SPBC106.15 | Ia | no deletion | lethal | YPL117c/IDI1 |
| SPBC106.16 | Ia | no deletion | lethal | YOL038w/PRE6 |
| SPBC106.17c | II | viable | viable | YNL277w/MET2 |
| SPBC106.18 | Ia | no deletion | lethal | YOL127w/RPL25 |
| SPBC106.19 | V | no deletion | — | |
| SPBC106.20 | Id | viable | lethal | YJL085w/EXO70 |
| SPBC582.03/cdc13 | Ib | lethal4 | viable | YPR119w/CLB2 |
| SPBC582.04c | V | viable | — | |
| SPBC582.05c/brc1 | Ib | viable5 | viable | YHR154w/ESC4 |
| SPBC582.06c | V | viable | — | |
| SPBC582.07c | Ib | lethal | lethal | YPR108w/RPN7 |
| SPBC582.08 | Ia | viable | viable | YDR111c |
| SPBC582.09 | IV | no deletion | — | |
| SPBC582.10c | Ia | viable | viable | YBR114w/RAD16 |
| SPBC582.11c | Ib | lethal | viable | YDL116w/NUP84 |
| SPBC582.12c | Ia | viable | viable | YJR148w/BAT2 |
| SPBC428.03c | Ib | viable | viable | YAR071w/PHO11 |
| SPBC428.04 | V | viable | — | |
| SPBC428.05c | Ia | viable | viable | YOL058w/ARG1 |
| SPBC428.06c | V | viable | — | |
| SPBC428.07 | V | no PCR* | — | |
| SPBC428.08c/clr4 | Ia | viable6 | viable | YJL168c/SET2 |
| SPBHC428.09c | V | viable | — | |
| SPBC428.10 | Ic | viable | viable | YIR019c/MUC1 |
| SPBC428.11 | Ia | viable | viable | YLR303w/MET17 |
| SPBC428.12c | Ib | viable | lethal | YER165w/PAB1 |
| SPBC428.13c/mob1 | Ib | lethal7 | lethal | YIL106w/MOB1 |
| SPBC428.14 | Ib | viable | viable | YBR042c |
| SPBC428.15 | Ia | no deletion | viable | YGR210c |
| SPBC428.16c/rhb1 | Ib | lethal8 | viable | YCR027c/RSG1 |
| SPBC428.17c | V | viable | — | |
| SPBC428.18/cdt1 | IV | lethal9 | — | |
| SPBC428.19c | Ib | viable | lethal | YMR093w |
| SPBC902.01c/alp6 | Ib | lethal10 | lethal | YNL126w/SPC98 |
| SPBC902.02c | Ia | viable | viable | YMR078c/CTF18 |
| SPBC902.03 | III | viable | viable | YAL009w/SPO7 |
| SPBC902.04 | IV | viable | — | |
| SPBC902.05c/glu2 | Ia | viable11 | viable | YOR136w/IDH2 |
| SPBC902.06 | V | viable | — | |
| SPBC1685.01/pmp1 | Ib | viable12 | viable | YIL113w |
| SPBC1685.02c | Ib | viable | viable | YOR369c/RPS12 |
| SPBC1685.03 | Ia | lethal | lethal | YIR022w/SEC11 |
| SPBC1685.04 | V | viable | — | |
| SPBC1685.05 | II | viable | viable | YNL123w |
| SPBC1685.06 | Ib | viable | viable | YNL299w/TRF5 |
| SPBC1685.07c | Ib | viable | viable | YBL089w |
| SPBC1685.08 | Ib | viable | viable | YPL181w |
| SPBC1685.09 | Ia | viable | viable | YLR388w/RPS29A |
| SPBC1685.10 | Ia | no deletion | viable | YHR021c/RPS27B |
| SPBC1685.11 | V | viable | — | |
| SPBC1685.12c | non- | coding ? | ||
| SPBC1685.13 | III | viable | viable | YPR149w/NCE102 |
| SPBC1685.14c | Ib | viable | viable | YNL212w/VID27 |
| SPBC649.01c/klp6 | Ib | viable13 | viable | YGL216w/KIP3 |
| SPBC649.02 | Ia | viable | viable | YNL302c/RPS19B |
| SPBC649.03 | Id | viable | viable | YMR201c/RAD14 |
| SPBC649.04/uvi15 | V | viable14 | — | |
| SPBC649.05/cut12 | V | lethal15 | — | |
| SPBC649.06 | Ia | viable | viable | YGR173w |
| SPBC354.02c/sec61 | Ia | lethal16 | lethal | YLR378c/SEC61 |
| SPBC354.03 | Ib | no PCR* | lethal | YBR198c/TAF90 |
| SPBC354.04 | V | no PCR* | — | |
| SPBC354.05c | Id | no deletion | viable | YOR032c/HMS1 |
| SPBC354.06 | Ia | no deletion | viable | YPL013c |
| SPBC354.07c | Ib | viable | viable | YPL145c/KES1 |
| SPBC354.08c | Ib | viable | viable | YMR266w |
| SPBC354.09c | Ib | viable | viable | YPL176c |
| SPBC354.10 | Ia | viable | viable | YIR019c/MUC1 |
| SPBC354.11c | V | viable | — | |
| SPBC354.12 | Ia | no deletion | viable | YJL052w/TDH1 |
| SPBC354.13 | Id | no deletion | viable | YDR389w/SAC7 |
| SPBC354.14c | Ib | viable | viable | YEL013w/VAC8 |
| SPBC354.15 | IV | viable | — | |
Fission yeast genes from chromosome II were deleted in a diploid strain using targeted PCR-based gene deletion procedure (Bähler et al., 1998). Diploids were sportulated and the spore viability was assessed on rich glucose medium. Deletion phenotype of the closest homolog in S. cerevisiae (if any) is given.
Yoshida et al. (1999), 2Yamashita et al. (1996), 3Fernandez et al. (1997), 4Booher and Beach (1988), 5Verkade et al. (1999), 6Bannister et al. (2001) and Nakayama et al. (2001), 7Hou et al. (2000), 8Mach et al. (2000), 9Hofmann and Beach (1994), 10Vardy and Toda (2000), 11Barel and MacDonald (1993), 12Sugiura et al. (1998) 13our study and West et al. (2002), 14Lee et al. (1995), 15Bridge et al. (1998), 16Broughton et al. (1997), 17YPD (see Garrels, 2002) and EUROSCARF (http://www.uni-frankfurt.de/fb15/mikro/euroscarf/). *No amplification of the deletion cassette.