Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov;141(21):4076–4086. doi: 10.1242/dev.108282

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 8. — Inhibition of mTOR suppresses proliferation of GSK3-deleted progenitors. (A) Top: dissociated neural progenitors from E13.5 GSK3-deficient mice actively proliferated in culture and formed neurospheres (arrows). Inhibition of mTOR activity by treatment with rapamycin suppressed neurosphere formation in GSK3-deleted progenitor culture. Bottom: GSK3-deleted progenitors were cultured and treated with BrdU. Proliferating cells positive for BrdU were reduced by rapymycin treatment. Scale bar: 80 μm. (B) Neurosphere size was quantified in GSK3-deficient cortical progenitors in the absence or presence of rapamycin. The treatment of GSK3-deleted cells with rapamycin decreased the diameter of neurospheres and the number of BrdU-labeled cells. *P<0.05. Data are mean±s.e.m. (C) Compared with control cells, GSK3-deficient neural progenitors generated more mitotic cells (phospho-histone H3) due to their self-renewal capacity. However, when treated with rapamycin, their proliferation capacity was suppressed. Scale bar: 40 μm. (D) Quantification of phospho-histone H3-positive cells. Control is Gsk3α^+/−; Gsk3β^loxP/+; Nestin-cre; knockout is Gsk3α^−/−; Gsk3β^loxP/loxP; Nestin-cre. *P<0.05. Data are mean±s.e.m. (E) Progenitors were transfected with control GFP, shGSK3, or shGSK3 and shRaptor. Transfection of shRaptor suppressed the proliferation of GSK3-deleted cells. Arrows and arrowheads indicate proliferating and non-proliferating cells from a whole transfected cells, respectively. Scale bar: 20 μm. (F) Quantification of E. *P<0.05. Data are mean±s.e.m.