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. 2014 Nov 15;141(22):4285–4297. doi: 10.1242/dev.110908

Fig. 8.

Fig. 8.

Msgn1 rescues paraxial mesoderm in Wnt3a−/− mutants. (A) Flow cytometry analysis of iF-Msgn1 or control iCre EBs treated with (+) or without (−) Dkk1 and/or Dox for 48 h for the expression of Pdgfrα and Flk1. The arrowheads indicate Pdgfrα+ cells. (B-G) β-Gal staining of E9.5 control (B), Wnt3a−/− (C), and Wnt3a−/−; Tcre-F-Msgn1GOF (D) mutants. (E-G) These panels represent corresponding cross sections of embryos depicted in B-D, taken at the indicated location (black line, B). Arrowheads indicate paraxial mesoderm. (H-K) WISH analysis of Meox1 in control (H), Tcre-F-Msgn1GOF (I), Wnt3a−/− (J), and Wnt3a−/−; Tcre-F-Msgn1GOF (K) mutants. Curved white line in J,K highlights the rescued posterior paraxial mesoderm domain. Scale bars: 200 μm (B,H), 100 μm (E).