Fig. 7.

MYR::UNC-5 inhibits growth cone filopodial protrusion similar to MYR::UNC-40. (A,B) Quantification of filopodia dynamics in VD growth cones as described in Fig. 2A. myr::unc-5 indicates a transgene that drives myr::unc-5 expression from the unc-25 promoter (see Materials and Methods and Fig. 1A). Two-sided t-tests with unequal variance were used to determine statistical significance. (C) Time-lapse series of a myr::unc-5 growth cone in an early L2 animal, taken at 4 min per frame. The arrowhead points to a growth cone with limited protrusion. (D) Time-lapse series of an unc-73(rh40); myr::unc-5 growth cone with long and persistent protrusions (arrowhead). Dorsal is up, and anterior is left. Scale bar: 5 µm.