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. 2014 Nov 15;141(22):4395–4405. doi: 10.1242/dev.110437

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — unc-73, unc-33 and unc-44 do not affect UNC-40::GFP or UNC-5::GFP accumulation in growth cones. (A) Fluorescence micrographs of MYR::UNC-40::GFP, full-length UNC-40::GFP and full-length UNC-5::GFP accumulation in VD growth cones in the indicated mutant backgrounds. Arrows point to growth cones. (B) Quantification of the mean pixel intensity of MYR::UNC-40::GFP, full-length UNC-40::GFP and full-length UNC-5::GFP in VD growth cones (see Materials and Methods). At least ten growth cones for each genotype were analyzed, except for unc-73(rh40); unc-5::gfp, which were subviable and sterile. unc-73(rh40); unc-5::gfp growth cones were not quantified, but those observed showed no gross change in UNC-5::GFP growth cone localization. (C) The average number of growth cone filopodia in different backgrounds (see Materials and Methods). At least ten growth cones were analyzed for each genotype. (B,C) Error bars represent s.e.m. Two-sided t-tests with unequal variance were used to determine statistical significance.