Fig. 2.

Adult adipocyte-specific knockdown of amino acid transporters does not cause obvious changes in ovarian or adipocyte morphology. (A,B) RT-PCR analysis of hand-dissected fat bodies showing knockdown of amino acid transporters (A), and normal ovariole and adipocyte morphology (B) after 10 days of Gal80^ts; Lsp2-mediated induction of RNAi or antisense transgenes against amino acid transporters or white control. Rp49 is a control. DAPI (white or blue) labels nuclei. In adipocytes, Nile Red, which fluoresces in both red and green (Greenspan et al., 1985), is shown in green. Scale bars: 100 μm (ovarioles); 10 μm (adipocytes).