Fig. 3.

Adult adipocyte-specific knockdown of a subset of amino acid transporters encoded by slif, CG7708, CG1607, CG13384, CG1628 and CG12773 results in reduced egg production. (A) Females carrying Gal80^ts; Lsp2 and UAS-RNAi transgenes against amino acid transporters [or a UAS-antisense transgene (Colombani et al., 2003) in the case of slif] or a control UAS-RNAi (against white, an eye color gene) raised at 18°C and switched to 29°C for adult adipocyte-specific knockdown for the indicated number of days. Knockdown of a subset of transporters causes a significant decrease in the average number of eggs laid per female per day. Transporters in red font were followed up on in this study. (B) Control females carrying UAS-RNAi or -antisense transgenes against amino acid transporters in the absence of Gal80^ts; Lsp2 and subjected to the same temperature regime as in A show egg-laying rates that are statistically indistinguishable from those of a UAS-GFP^RNAi control, except for UAS-CG13384^RNAi. *P<0.05; **P<0.01; ***P<0.001, Student's t-test. Data shown as mean±s.e.m.