Fig. 4.
Adult adipocyte-specific knockdown of amino acid transporters leads to increased rates of GSC loss in the ovary. (A) Average number of GSCs per germarium at 0, 5 or 10 days of Gal80ts; Lsp2-mediated induction of RNAi or antisense transgenes against amino acid transporters or white control. See supplementary material Fig. S3 for sample sizes and distribution. **P<0.01; ****P<0.0001, two-way ANOVA with interaction. Data shown as mean±s.e.m. (B) Germaria at 10 days of adipocyte-specific GFP control or amino acid transporter RNAi labeled for phosphorylated Mad (pMad; green), 1B1 (red, fusome) and Lamin C (red, cap cell nuclear envelope). GSC nuclei are outlined. Scale bar: 2.5 μm. (C) Box and whisker plot of mean nuclear pMad intensity for experiment in B. Sample sizes are included above. **P<0.01; ***P<0.001, Student's t-test.