Fig. 5.

Decreased fertility of Neat1 KO mice is rescued by progesterone administration. (A) Beeswarm boxplot of the number of implanted embryos in WT, Neat1 KO and progesterone-administered Neat1 KO (Rescue) mice at 14.5 dpc. All of the mice given progesterone became pregnant. Each blue dot represents an individual mouse. Boxes represent the mean, 25th and 75th percentiles; whiskers show the maximum and minimum. (B) The uterus of a Neat1 KO mouse implanted with progesterone pellets at 14.5 dpc. (C) Daily record of copulation of Neat1 KO mice transplanted with progesterone pellets. The arrowhead indicates the day of transplantation. Each black box represents the day at which plug formation was observed. Each row represents an individual animal. (D) Histological and in situ hybridization analysis of the corpus luteum of WT and Neat1 KO mice transplanted with progesterone pellets. Note the slightly shrunken cytoplasm of the Neat1 KO luteal cells. The expression of Star, Hsd3b and Vegfa was slightly decreased in the Neat1 KO mice, whereas the expression of Cyp11a1 was not affected. Scale bars: 10 µm (HE staining), 100 µm (other panels). P values were calculated using a two-tailed, nonequal variance t-test.