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. 2015 Jan 22;5:223. doi: 10.3389/fendo.2014.00223

Figure 1.

Figure 1

Insulin resistant 3T3-F442a adipocytes display altered adipocytokine expression. (A) 3T3-F442a adipocytes were grown under insulin responsive (LG) or insulin resistant (LG with PUGNAc, LG with GlcNAcstatin, or HG with insulin) conditions as described in Section “Materials and Methods.” Equal amounts of protein from whole cell lysates were separated by SDS-PAGE and Western blotting was performed using anti-O-GlcNAc (CTD110.6). Equal loading was confirmed by Western blotting with ERK2. (B) A partial list of rodent adipocytokines found to be regulated by insulin resistance based on proteomic quantification. +Indicates that the protein expression is upregulated upon the induction of insulin resistance. (C) Proteomic quantification of protein expression was confirmed by Western blotting in 3T3-F442a adipocytes. Equal amounts of concentrated media were separated by SDS-PAGE and Western blotting was performed with the designated antibodies. (D) The steady-state transcript levels were evaluated using qPCR in 3T3-F442a adipocytes. Data are presented so that 100% represents the transcript level in the insulin responsive condition (LG). *P < 0.05.