Table 1.
Summary of experimental conditions (control, hydrogen- and nutrient-limited treatments) and respective cell concentrations (×108 cells ml-1, in parenthesis the SD) of Methanothermobacter thermautotrophicus at: late exponential phase (L-Exp), early, mid, and late stationary phases (E-, M-, and L-Stat).
| Control | Hydrogen-limited | Nutrient-limited | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Headspace | 80:20 (H2:CO2 vol:vol) | 80:20 (H2:CO2) until L-Exp and | 80:20 (H2:CO2 vol:vol) | ||||||||
| 80:20 (N2:CO2) 3 h prior to E-Stat | |||||||||||
| Nutrients | 4.3 and 3.0 mM | 4.3 and 3.0 mM | 20 and 10 μM | ||||||||
| L-Exp | E-Stat | M-Stat | L-Stat | L-Exp | E-Stat | M-Stat | L-Stat | L-Exp | E-Stat | L-Stat | |
| Cell | 1.0 | 6.9 | 5.7 | 6.0 | 1.6 | 5.0 | 5.1 | 4.9 | 0.1 | 0.2 | 0.2 |
| concentrations | (0.8) | (1.1) | (0.7) | (0.8) | (0.3) | (0.2) | (0.2) | (0.1) | (0.09) | (0.01) | (0.01) |
Headspace gas atmosphere (vol:vol) and concentrations of potassium and phosphate, respectively.