Table 2.
Sequence alignment of AcrB with homologes at sites that restored AcrBP223G activity.
| Residue number | 199 | 209 | 256 | 257 | 662 | 737 | 788 | 800 | 810 |
|---|---|---|---|---|---|---|---|---|---|
| AcrB(E. coli) | T | A | D | G | M | Q | D | P | E |
| Replaced by | M | V | N | V | I | L | K | S | K |
| AcrD (E. coli) | T | S | D | G | V | L | Y | S | G |
| AcrF (E. coli) | T | V | D | G | V | A | L | F | Y |
| MexB (P. aeruginosa) | T | A | D | G | M | A | W | F | Y |
| AcrB(H. pylori) | D | V | I | G | V | Q | E | R | P |
| AcrB(Y. pestis) | T | I | D | G | V | A | W | F | Y |
| AcrB(V. cholera) | V | S | G | D | Q | I | W | Q | E |
| AcrB(K. pneumonia) | T | A | D | G | V | A | W | F | Y |
| AdeB (A. baumannii) | L | R | T | N | P | D | G | Q | R |
| AmeB (A. tumefaciens) | T | Q | G | A | I | D | N | S | S |
| TtgB (P. Putita) | T | A | D | G | V | L | Y | S | G |
| CmeB (C. coli) | G | V | N | E | G | M | N | T | V |
| AmrB (P. aeruginosa) | A | H | G | G | N | M | E | K | I |
| MtrD (N. meningitidis) | A | N | S | N | V | G | V | T | T |
| Consensus score | 3 | 2 | 3 | 4 | 4 | 3 | 2 | 2 | 0 |
Replacement residues identified in suppressor mutants were also shown.