FIG. 5.

Effect of anti-CD4 upon AAV vector readministration. GAA-KO mice were treated with AAV2/9-CBhGAApA with (+) anti-CD4 (3 times) or without (−) anti-CD4 pretreatment. At week 20, mice were injected with AAV2/8-LSPGFP (n=6 in each group), and euthanized at week 24. Liver was frozen and sectioned to detect GFP expression using confocal microscopy. Images from three different animals in each group are shown (A). The pixel intensity was analyzed with ImageJ (B). The quantity of vector genomes was also analyzed by qPCR (C). *p<0.05; ***p<0.001. Color images available online at www.liebertpub.com/hum