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. 2014 Sep 17;24(3):284–295. doi: 10.1089/scd.2014.0210

FIG. 1.

FIG. 1.

Effect of emodin on self-renewal activity of glioma stem cells (GSCs). (A) Sphere-forming assays of GSCs. Cells (1×103 cells/mL) were cultured in serum-free Dulbecco's modified Eagle's medium (DMEM)/F12 medium with growth factors (20 ng/mL of epidermal growth factor and 10 ng/mL of basic fibroblast growth factor) for 10 days and counted the spheres. Representative images of spheres (upper, magnification, ×40) and quantification of the assays (lower). (B) Limiting dilution assays for measuring self-renewal capacity of GSCs. (C) Soft agar colony-forming assays for evaluating tumorigenic capacity of GSCs in vitro. Detailed experimental procedures were described in the section “Materials and Methods.” *P<0.05, **P<0.01, ***P<0.005.

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