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Effect of emodin on the self-renewal associated signaling pathways in GSCs. Western blot analysis of GSCs. Cells were treated with or without emodin at the indicated concentrations on the figure for 24 h (A) or 5 μM of emodin at the indicated time points on (B, C). Total cells were lysed in a lysis buffer and subjected to western blot using antibodies described in the figure. β-Actin was used as a loading control.
