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. 2014 Sep 24;16:441. doi: 10.1186/s13058-014-0441-7

Figure 1.

Figure 1

Differential P-REX1 expression and methylation of the PREX1 gene promoter in mammary cell lines. Panel A. P-REX1 levels in human breast cell lines were determined by Western blot. β-actin was used as loading control. N, normal; B, basal-like; L, luminal. Panel B. Quantitative PCR determination of P-REX1 mRNA levels in human breast cancer cell lines, normalized to those in T-47D cells. B2M and UBC were used as housekeeping genes. Data are expressed as mean ± S.D. of triplicate samples. A second additional experiment gave similar results. As P-REX1 mRNA levels in MCF-10A are non detectable (ND), statistical analysis is not provided. Panel C. Schematic representation of the PREX1 gene promoter. The location of the two CpG islands, the four regions amplified by BSPs, and the ATG codon are indicated. Panel D. DNA methylation of the PREX1 promoter was determined by bisulfite sequencing PCR. Each dot represents the methylation status of a CpG dinucleotide in one sequenced BSP clone. Black dot, methylated CpG; white dot, unmethylated CpG.