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. 2014 May 8;3(4):812–824. doi: 10.1002/cam4.219

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© 2014 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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In vitro and in vivo growth U-251 clones. (A) Growth curves of U-251MG in MEM, U-251MG in DMEM, U-251-4q12, U-251N, and U251-FGA20gain. (B) BrdU incorporation shows various distributions of the S-phase cells. One representative figure is shown, while numbers are the mean from three replicate analyses. (C) Examples of variation in colony formation obtained by U-251MG (left), U-251-4q12 (middle left), U-251N (middle right), and U-251-FGA20gain (right). Single colonies are shown as insets with scale bars = 100 μm. (D) Survival of clonogenic colonies after irradiation at 2, 5, and 10 Gy. U-251-4q12, U-251N, and U-251-FGA20gain. (E) Kaplan-Meier survival curves after intracranial implantation of the U-251 subclones in NOD/SCID mice.