Fig. 2.

GZL decreases the expression of NF-κB subunits p65 and p50 in (A) absence and (B) presence of TNFα treatment. The effects were compared to the negative control (N) and to the positive control, rocaglamide (R). Western blot analysis was performed on whole cell HeLa extracts and showed that a decrease in activity was detected upon higher concentrations of GZL. (C) IKKα and IKKβ were down-regulated in treated HeLa cells. β-Actin expression was used as an internal control to establish equivalent protein loading.