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GZL decreases the expression of NF-κB mediators in HT-29 cells. GZL suppresses NF-κB activation in comparison with the negative control (N) and the positive control rocaglamide (R), in the presence of TNFα treatment. (A) A decrease in NF-κB p65 and p50 subunits was detected at different concentrations of GZL. (B) IKKα and IKKβ were downregulated in treated HT-29 cells. β-Actin expression was used as an internal control to establish equivalent protein loading.
